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Image Search Results
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: Differential role of IFN-gamma-inducible protein 10 kDa in a cockroach antigen-induced model of allergic airway hyperreactivity: systemic versus local effects.
doi: 10.4049/jimmunol.169.12.7045
Figure Lengend Snippet: FIGURE 7. CXCR3 expression on airway eo- sinophils. Airway cells isolated via BAL from mice challenged with IP10 and CRA IT were cytospun and stained immunohistochemically for the expres- sion of CXCR3 (A); isotype control (1), anti- murine CXCR3 (2–4), and peripheral blood cells (5 and 6) isolated from S. mansoni-infected mice were cytospun and stained for CXCR3 expression (isotype control and anti-murine CXCR3, respec- tively). Arrows indicate positively stained eosino- phils and the asterisk indicates a negatively stained neutrophil. Eosinophils were isolated and purified from S. mansoni-infected mice and stained flow cy- tometrically for the expression of CCR3 and CXCR3, a representative dot plot is shown in B. Airway cells isolated from the BAL from mice 24 h post IT challenge with CRA or IP10 and CRA were stained flow cytometrically, using the same gating parameters as with the purified eosinophils (Ba) to detect CCR3 and CXCR3 expressing cells (C). Dot plots shown are representative of n 3, the num- bers listed in each quadrant indicate the mean total number of cells in each cellular population calcu- lated from the total number of cells recovered from the BAL fluid. , p 0.05.
Article Snippet: The cytospins were stained using and anti-goat HRP-3-amino-9-ethylcarbazole immunostaining kit (R&D Systems) along with
Techniques: Expressing, Isolation, Staining, Control, Infection